Human COLEC11 Recombinant Protein (N-His) (HA145012)

Name: Human COLEC11 Recombinant Protein (N-His)
Brand: abinScience
SKU: HA145012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HA145012
Description	Recombinant Human COLEC11 Protein, N-His (HA145012) expressed in E. coli, spanning Ser151-Met271. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q9BWP8
Protein length	Ser151-Met271
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	15.85 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Collectin-11, Collectin kidney protein 1, CL-K1, COLEC11
Background	Collectin-11 (COLEC11) is a ~28 kDa protein. Lectin that plays a role in innate immunity, apoptosis and embryogenesis. Calcium-dependent lectin that binds self and non-self glycoproteins presenting high mannose oligosaccharides with at least one terminal alpha-1,2-linked mannose epitope. Primarily recognizes the terminal disaccharide of the glycan. Also recognizes a subset of fucosylated glycans and lipopolysaccharides. Plays a role in innate immunity through its ability to bind non-self sugars presented by microorganisms and to activate the complement through the recruitment of MAPS1. 1. Rooryck, C. et al. (2011) Nature genetics 43, 197-203. PMID: 21258343 2. Henriksen, ML. et al. (2013) Molecular immunology 56, 757-67. PMID: 23954398 3. Venkatraman Girija, U. et al. (2015) BMC biology 13, 27. PMID: 25912189 4. Keshi, H. et al. (2006) Microbiology and immunology 50, 1001-13. PMID: 17179669 5. Hansen, S. et al. (2010) Journal of immunology (Baltimore, Md. : 1950) 185, 6096-104. PMID: 20956340 6. Munye, MM. et al. (2017) PLoS genetics 13, e1006679. PMID: 28301481
Note	For research use only.

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SDS-PAGE for Recombinant Human COLEC11 Protein, N-His

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HA145012-SDS-PAGE-1.jpg
Multi-omics analysis reveals the interaction between the complement system and the coagulation cascade in the development of endometriosis

Datasheet

Human COLEC11 Recombinant Protein (N-His).pdf

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Human COLEC11 Recombinant Protein (N-His) (HA145012)

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