Human EIF3E Recombinant Protein (N-His) (HD106012)

Name: Human EIF3E Recombinant Protein (N-His)
Brand: abinScience
SKU: HD106012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HD106012
Description	Recombinant Human EIF3E Protein, N-His (HD106012) expressed in E. coli, spanning Met1-Tyr445. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P60228
Protein length	Met1-Tyr445
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	54.38 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	EIF3E, EIF3S6, Eukaryotic translation initiation factor 3 subunit 6, Eukaryotic translation initiation factor 3 subunit E, INT6, Viral integration site protein INT-6 homolog, eIF-3 p48, eIF3e
Background	Eukaryotic translation initiation factor 3 subunit E is a ~52 kDa protein. Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression. 1. Masutani, M. et al. (2007) The EMBO journal 26, 3373-83. PMID: 17581632 2. Lee, AS. et al. (2015) Nature 522, 111-4. PMID: 25849773 3. Lee, AS. et al. (2016) Nature 536, 96-9. PMID: 27462815 4. Morris, C. et al. (2007) EMBO reports 8, 596-602. PMID: 17468741 5. Buchsbaum, S. et al. (2007) Oncogene 26, 5132-44. PMID: 17310990 6. Chen, L. et al. (2007) The Journal of biological chemistry 282, 12707-16. PMID: 17324924
Note	For research use only

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Datasheet

Human EIF3E Recombinant Protein (N-His).pdf

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Human EIF3E Recombinant Protein (N-His) (HD106012)

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