Human ELANE Recombinant Protein (N-His) (HY338022)

Name: Human ELANE Recombinant Protein (N-His)
Brand: abinScience
SKU: HY338022
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HY338022
Description	Recombinant Human ELANE Protein, N-His (HY338022) expressed in E. coli. Purity: >90% as determined by SDS-PAGE.. Highlights ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% as determined by SDS-PAGE.
Expression system	E. coli
Accession	P08246
Protein length	Ile30-Gln247
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	25.58 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Bone marrow serine protease, EC:3.4.21.37, ELA2, ELANE, Elastase-2, HLE, Human leukocyte elastase, Medullasin, Neutrophil elastase, PMN elastase
Background	Neutrophil elastase is a ~28 kDa protein. Serine protease that modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis. Promotes cleavage of GSDMB, thereby inhibiting pyroptosis. Promotes blood coagulation. Through the activation of the platelet fibrinogen receptor integrin alpha-IIb/beta-3, potentiates platelet aggregation induced by a threshold concentration of cathepsin G (CTSG). 1. Tralau, T. et al. (2004) Experimental dermatology 13, 316-25. PMID: 15140022 2. Oltra, SS. et al. (2023) Cell death and differentiation 30, 1366-1381. PMID: 36899106 3. Massberg, S. et al. (2010) Nature medicine 16, 887-96. PMID: 20676107 4. Waisberg, M. et al. (2014) PLoS pathogens 10, e1004338. PMID: 25211214 5. Si-Tahar, M. et al. (1997) The Journal of biological chemistry 272, 11636-47. PMID: 9111081 6. Belaaouaj, A. et al. (2000) Science (New York, N.Y.) 289, 1185-8. PMID: 10947984
Note	For research use only

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SDS-PAGE for Recombinant Human ELANE Protein, N-His

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Datasheet

Human ELANE Recombinant Protein (N-His).pdf

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Human ELANE Recombinant Protein (N-His) (HY338022)

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