ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Homo sapiens (Human)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Predicted molecular weight
33.38 kDa
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names
BTF2 p80, Basic transcription factor 2 80 kDa subunit, CXPD, DNA 5'-3' helicase XPD, DNA excision repair protein ERCC-2, DNA repair protein complementing XP-D cells, EC:5.6.2.3, ERCC2, General transcription and DNA repair factor IIH helicase subunit XPD, TFIIH 80 kDa subunit, TFIIH basal transcription factor complex 80 kDa subunit, TFIIH p80, TFIIH subunit XPD, XPD, XPDC, Xeroderma pigmentosum group D-complementing protein
Background
General transcription and DNA repair factor IIH helicase subunit XPD is a ~86 kDa protein. ATP-dependent 5'-3' DNA helicase. Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, not absolutely essential for minimal transcription in vitro. Required for transcription-coupled nucleotide excision repair (NER) of damaged DNA; recognizes damaged bases. Sequestered in chromatin on UV-damaged DNA. When complexed to CDK-activating kinase (CAK), involved in transcription by RNA polymerase II.
1. Kokic, G. et al. (2019) Nature communications 10, 2885. PMID: 31253769
2. Sung, P. et al. (1993) Nature 365, 852-5. PMID: 8413672
3. Coin, F. et al. (1998) Nature genetics 20, 184-8. PMID: 9771713
4. Tirode, F. et al. (1999) Molecular cell 3, 87-95. PMID: 10024882
5. Coin, F. et al. (2007) Molecular cell 26, 245-56. PMID: 17466626
6. Mathieu, N. et al. (2013) Current biology : CB 23, 204-12. PMID: 23352696
7. Aibara, S. et al. (2021) Nature 594, 124-128. PMID: 33902107
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