Human FXR1 Recombinant Protein (N-His) (HW827012)

Name: Human FXR1 Recombinant Protein (N-His)
Brand: abinScience
SKU: HW827012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HW827012
Expression system	E. coli
Accession	P51114
Protein length	Met1-Ala173
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Fragile X mental retardation syndrome-related protein 1, hFXR1p, FXR1
Background	RNA-binding protein FXR1 is a ~69 kDa protein. mRNA-binding protein that acts as a regulator of mRNAs translation and/or stability, and which is required for various processes, such as neurogenesis, muscle development and spermatogenesis. Specifically binds to AU-rich elements (AREs) in the 3'-UTR of target mRNAs. Promotes formation of some phase-separated membraneless compartment by undergoing liquid-liquid phase separation upon binding to AREs-containing mRNAs, leading to assemble mRNAs into cytoplasmic ribonucleoprotein granules that concentrate mRNAs with associated regulatory factors. Required to activate translation of stored mRNAs during late spermatogenesis: acts by undergoing liquid-liquid phase separation to assemble target mRNAs into cytoplasmic ribonucleoprotein granules that recruit translation initiation factor EIF4G3 to activate translation of stored mRNAs in late spermatids. Promotes translation of MYC transcripts by recruiting the eIF4F complex to the translation start site. 1. Vasudevan, S. et al. (2007) Cell 128, 1105-18. PMID: 17382880 2. Say, E. et al. (2010) Molecular cell 38, 236-49. PMID: 20417602 3. Herman, AB. et al. (2018) Cell reports 24, 1176-1189. PMID: 30067974 4. George, J. et al. (2021) Cell reports 37, 109934. PMID: 34731628 5. Ling, H. et al. (2022) Oncogene 41, 4433-4445. PMID: 35989368 6. Datta, C. et al. (2022) Science advances 8, eabo1304. PMID: 36306353 9. Fischer-Kešo, R. et al. (2014) Molecular and cellular biology 34, 4244-56. PMID: 25225333
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human FXR1 Protein, N-His

References

HW827012-SDS-PAGE-1.jpg

Datasheet

Human FXR1 Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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C₁ × V₁ = C₂ × V₂

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Stock Solution

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Working Solution

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Human FXR1 Recombinant Protein (N-His) (HW827012)

Overview

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References

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