Human GRIK2 Recombinant Protein (N-His) (HC267012)

Name: Human GRIK2 Recombinant Protein (N-His)
Brand: abinScience
SKU: HC267012
Price: 211 USD
Availability: InStock

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Catalog No.	HC267012
Description	Recombinant Human GRIK2 Protein, N-His (HC267012) expressed in E. coli, spanning Asn286-Pro561. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q13002
Protein length	Asn286-Pro561
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	33.3 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	EAA4, GluR6, Glutamate receptor 6, Excitatory amino acid receptor 4, Glutamate receptor ionotropic, kainate 2, GluK2, GLUR6, GRIK2, GluR-6
Background	Glutamate receptor ionotropic, kainate 2 (GRIK2) is a ~102 kDa protein. Ionotropic glutamate receptor that functions as a cation-permeable ligand-gated ion channel, gated by L-glutamate and the glutamatergic agonist kainic acid. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformational change leading to the opening of the cation channel, converting the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. Modulates cell surface expression of NETO2. 1. Hoo, KH. et al. (1994) Receptors & channels 2, 327-37. PMID: 7536611 2. Swanson, GT. et al. (1996) The Journal of physiology 492 ( Pt 1), 129-42. PMID: 8730589 3. Hayes, DM. et al. (2003) Biochemical and biophysical research communications 310, 8-13. PMID: 14511640 4. Guzmán, YF. et al. (2017) Neurology. Genetics 3, e129. PMID: 28180184 5. Stolz, JR. et al. (2021) American journal of human genetics 108, 1692-1709. PMID: 34375587
Note	For research use only.

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SDS-PAGE for Recombinant Human GRIK2 Protein, N-His

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Datasheet

Human GRIK2 Recombinant Protein (N-His).pdf

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Human GRIK2 Recombinant Protein (N-His) (HC267012)

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