Human HEPC/Hepcidin Recombinant Protein (N-GST & C-His) (HX911022)

Name: Human HEPC/Hepcidin Recombinant Protein (N-GST & C-His)
Brand: abinScience
SKU: HX911022
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HX911022
Description	Recombinant Human HEPC/Hepcidin Protein, N-GST & C-His (HX911022) expressed in E. coli, spanning Ser25-Thr84. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P81172
Protein length	Ser25-Thr84
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	35.12 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Putative liver tumor regressor, HEPC, LEAP-1, Liver-expressed antimicrobial peptide 1, PLTR, Hepcidin, LEAP1, Hepc25, Hepc20, HAMP
Background	Hepcidin is a ~9 kDa protein. Liver-produced hormone that constitutes the main circulating regulator of iron absorption and distribution across tissues. Acts by promoting endocytosis and degradation of ferroportin/SLC40A1, leading to the retention of iron in iron-exporting cells and decreased flow of iron into plasma. Controls the major flows of iron into plasma: absorption of dietary iron in the intestine, recycling of iron by macrophages, which phagocytose old erythrocytes and other cells, and mobilization of stored iron from hepatocytes. 1. Qiao, B. et al. (2012) Cell metabolism 15, 918-24. PMID: 22682227 2. Aschemeyer, S. et al. (2018) Blood 131, 899-910. PMID: 29237594 3. Billesbølle, CB. et al. (2020) Nature 586, 807-811. PMID: 32814342 4. Ganz, T. et al. (2012) Biochimica et biophysica acta 1823, 1434-43. PMID: 22306005
Note	For research use only.