ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Homo sapiens (Human)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Predicted molecular weight
16.19 kDa
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names
Interferon regulatory factor 1, IRF-1, IRF1
Background
Interferon regulatory factor 1 (IRF1) is a ~36 kDa protein. Transcriptional regulator which displays a remarkable functional diversity in the regulation of cellular responses. Regulates transcription of IFN and IFN-inducible genes, host response to viral and bacterial infections, regulation of many genes expressed during hematopoiesis, inflammation, immune responses and cell proliferation and differentiation, regulation of the cell cycle and induction of growth arrest and programmed cell death following DNA damage. Stimulates both innate and acquired immune responses through the activation of specific target genes and can act as a transcriptional activator and repressor regulating target genes by binding to an interferon-stimulated response element (ISRE) in their promoters. Has an essentail role in IFNG-dependent immunity to mycobacteria. Competes with the transcriptional repressor ZBED2 for binding to a common consensus sequence in gene promoters.
1. Oshima, S. et al. (2004) Molecular and cellular biology 24, 6298-310. PMID: 15226432
2. Dornan, D. et al. (2004) Molecular and cellular biology 24, 10083-98. PMID: 15509808
3. Su, ZZ. et al. (2007) Journal of cellular physiology 213, 502-10. PMID: 17516545
4. Park, J. et al. (2007) Proceedings of the National Academy of Sciences of the United States of America 104, 17028-33. PMID: 17942705
5. Bowie, ML. et al. (2008) Advances in experimental medicine and biology 617, 367-74. PMID: 18497060
6. Huang, Y. et al. (2009) PloS one 4, e5397. PMID: 19404407
7. Gao, J. et al. (2010) Cell death and differentiation 17, 699-709. PMID: 19851330
8. Qi, H. et al. (2012) The Journal of biological chemistry 287, 12622-33. PMID: 22367195
9. Somerville, TDD. et al. (2020) Proceedings of the National Academy of Sciences of the United States of America 117, 11471-11482. PMID: 32385160
10. Tanaka, N. et al. (1993) Molecular and cellular biology 13, 4531-8. PMID: 7687740
Note
For research use only.
Images
SDS-PAGE
SDS-PAGE for Recombinant Human IRF1 Protein, N-His
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