ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Homo sapiens (Human)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Predicted molecular weight
36.42 kDa
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names
Bifunctional arginine demethylase and lysyl-hydroxylase JMJD6, EC:1.14.11.-, Histone arginine demethylase JMJD6, JMJD6, JmjC domain-containing protein 6, Jumonji domain-containing protein 6, KIAA0585, Lysyl-hydroxylase JMJD6, PSR, PTDSR, Peptide-lysine 5-dioxygenase JMJD6, Phosphatidylserine receptor, Protein PTDSR
Background
Bifunctional arginine demethylase and lysyl-hydroxylase JMJD6 is a ~46 kDa protein. Dioxygenase that can both act as a arginine demethylase and a lysyl-hydroxylase. Acts as a lysyl-hydroxylase that catalyzes 5-hydroxylation on specific lysine residues of target proteins such as U2AF2/U2AF65 and LUC7L2. Regulates RNA splicing by mediating 5-hydroxylation of U2AF2/U2AF65, affecting the pre-mRNA splicing activity of U2AF2/U2AF65. Hydroxylates its own N-terminus, which is required for homooligomerization. Plays a role in the regulation of nucleolar liquid-liquid phase separation (LLPS) by post-translationally modifying LIAT1 at its lysine-rich domain which inhibits LIAT1 nucleolar targeting.
1. Chang, B. et al. (2007) Science (New York, N.Y.) 318, 444-7. PMID: 17947579
2. Mantri, M. et al. (2010) Journal of molecular biology 401, 211-22. PMID: 20684070
3. Hahn, P. et al. (2010) PloS one 5, e13769. PMID: 21060799
4. Han, G. et al. (2012) Journal of cellular biochemistry 113, 1663-70. PMID: 22189873
5. Poulard, C. et al. (2014) PloS one 9, e87982. PMID: 24498420
6. Webby, CJ. et al. (2009) Science (New York, N.Y.) 325, 90-3. PMID: 19574390
7. Hong, X. et al. (2010) Proceedings of the National Academy of Sciences of the United States of America 107, 14568-72. PMID: 20679243
8. Konuma, T. et al. (2017) Scientific reports 7, 16272. PMID: 29176719
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