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Human KHDRBS3 Recombinant Protein (N-His) (HD548012)

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Overview
Catalog No.HD548012
Description
Recombinant Human KHDRBS3 Protein, N-His (HD548012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE..
Highlights
  • E. coli Expression — High-yield, cost-effective production.
  • High Purity — >90% as determined by SDS-PAGE.
Expression systemE. coli
AccessionO75525
Protein lengthAsn51-Gly176
ApplicationsELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
SpeciesHomo sapiens (Human)
Nature Recombinant
Endotoxin level Please contact with the lab for this information.
Purity >90% as determined by SDS-PAGE.
Form Lyophilized
Storage buffer Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate NamesKH domain-containing, RNA-binding, signal transduction-associated protein 3, KHDRBS3, RNA-binding protein T-Star, SALP, SLM-2, SLM2, Sam68-like mammalian protein 2, Sam68-like phosphotyrosine protein
Background

KH domain-containing, RNA-binding, signal transduction-associated protein 3 is a ~38 kDa protein. RNA-binding protein that plays a role in the regulation of alternative splicing and influences mRNA splice site selection and exon inclusion. Binds preferentially to the 5'-[AU]UAAA-3' motif in vitro. Binds optimally to RNA containing 5'-[AU]UAA-3' as a bipartite motif spaced by more than 15 nucleotides. Binds poly(A). RNA-binding abilities are down-regulated by tyrosine kinase PTK6.

1. Lee, J. et al. (1999) Gene 240, 133-47. PMID: 10564820
2. Ray, D. et al. (2009) Nature biotechnology 27, 667-70. PMID: 19561594
3. Feracci, M. et al. (2016) Nature communications 7, 10355. PMID: 26758068
4. Cohen, CD. et al. (2005) Journal of the American Society of Nephrology : JASN 16, 1958-65. PMID: 15901763
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
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Stock Solution
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V₁ (Stock Vol.)
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Working Solution
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