Human LCAT Recombinant Protein (N-His) (HY349012)

Name: Human LCAT Recombinant Protein (N-His)
Brand: abinScience
SKU: HY349012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HY349012
Description	Recombinant Human LCAT Protein, N-His (HY349012) expressed in E. coli, spanning Phe25-Glu440. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P04180
Protein length	Phe25-Glu440
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	49.38 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Platelet-activating factor acetylhydrolase, Phosphatidylcholine-sterol acyltransferase, Phospholipid-cholesterol acyltransferase, PAF acetylhydrolase, LCAT, 1-alkyl-2-acetylglycerophosphocholine esterase, Lecithin-cholesterol acyltransferase
Background	Phosphatidylcholine-sterol acyltransferase (LCAT) is a ~49 kDa protein. Central enzyme in the extracellular metabolism of plasma lipoproteins. Synthesized mainly in the liver and secreted into plasma where it converts cholesterol and phosphatidylcholines (lecithins) to cholesteryl esters and lysophosphatidylcholines on the surface of high and low density lipoproteins (HDLs and LDLs). The cholesterol ester is then transported back to the liver. Has a preference for plasma 16:0-18:2 or 18:O-18:2 phosphatidylcholines. Also produced in the brain by primary astrocytes, and esterifies free cholesterol on nascent APOE-containing lipoproteins secreted from glia and influences cerebral spinal fluid (CSF) APOE- and APOA1 levels. 1. Kosek, AB. et al. (1999) Biochemical and biophysical research communications 258, 548-51. PMID: 10329423 2. Hirsch-Reinshagen, V. et al. (2009) Journal of lipid research 50, 885-93. PMID: 19065001 3. Piper, DE. et al. (2015) Journal of lipid research 56, 1711-9. PMID: 26195816 4. Subbaiah, PV. et al. (1996) Journal of lipid research 37, 113-22. PMID: 8820107 5. Clay, MA. et al. (2000) The Journal of biological chemistry 275, 9019-25. PMID: 10722751 6. Liu, M. et al. (1994) Proceedings of the National Academy of Sciences of the United States of America 91, 6035-9. PMID: 8016111 7. La Marca, V. et al. (2014) Journal of neurochemistry 130, 97-108. PMID: 24620755
Note	For research use only.

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SDS-PAGE for Recombinant Human LCAT Protein, N-His

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Datasheet

Human LCAT Recombinant Protein (N-His).pdf

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Human LCAT Recombinant Protein (N-His) (HY349012)

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