Human MAPK10 Recombinant Protein (N-His) (HX886012)

Name: Human MAPK10 Recombinant Protein (N-His)
Brand: abinScience
SKU: HX886012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HX886012
Description	Recombinant Human MAPK10 Protein, N-His (HX886012) expressed in E. coli, spanning Arg88-Ala332. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P53779
Protein length	Arg88-Ala332
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	30.67 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	MAPK10, Mitogen-activated protein kinase 10, Stress-activated protein kinase 1b, c-Jun N-terminal kinase 3, Stress-activated protein kinase JNK3, MAP kinase 10, MAP kinase p49 3F12, JNK3, SAPK1B, PRKM10, MAPK 10, SAPK1b, JNK3A
Background	Mitogen-activated protein kinase 10 (MAPK10) is a ~52 kDa protein. Serine/threonine-protein kinase involved in various processes such as neuronal proliferation, differentiation, migration and programmed cell death. Extracellular stimuli such as pro-inflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK10/JNK3. In turn, MAPK10/JNK3 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN and ATF2 and thus regulates AP-1 transcriptional activity. Plays regulatory roles in the signaling pathways during neuronal apoptosis. 1. Yoshitane, H. et al. (2012) EMBO reports 13, 455-61. PMID: 22441692 2. Huang, J. et al. (2012) Nature 482, 542-6. PMID: 22327296
Note	For research use only.

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SDS-PAGE for Recombinant Human MAPK10 Protein, N-His

References

HX886012-SDS-PAGE-1.jpg

Datasheet

Human MAPK10 Recombinant Protein (N-His).pdf

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Human MAPK10 Recombinant Protein (N-His) (HX886012)

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