Human MCIDAS Recombinant Protein (N-GST & C-His) (HA153012)

Name: Human MCIDAS Recombinant Protein (N-GST & C-His)
Brand: abinScience
SKU: HA153012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HA153012
Description	Recombinant Human MCIDAS Protein, N-GST & C-His (HA153012) expressed in E. coli, spanning Thr314-Ser385. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	D6RGH6
Protein length	Thr314-Ser385
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	36.13 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Multicilin, Multiciliate differentiation and DNA synthesis-associated cell cycle protein, McIdas protein, Protein Idas, MCIDAS, IDAS, MCI, MCIN
Background	Multicilin (MCIDAS) is a ~41 kDa protein. Transcription regulator specifically required for multiciliate cell differentiation. Acts in a multiprotein complex containing E2F4 and E2F5 that binds and activates genes required for centriole biogenesis. Required for the deuterosome-mediated acentriolar pathway. Plays a role in mitotic cell cycle progression by promoting cell cycle exit. Modulates GMNN activity by reducing its affinity for CDT1. 1. Boon, M. et al. (2014) Nature communications 5, 4418. PMID: 25048963 2. Pefani, DE. et al. (2011) The Journal of biological chemistry 286, 23234-46. PMID: 21543332 3. Caillat, C. et al. (2013) The Journal of biological chemistry 288, 31624-34. PMID: 24064211
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human MCIDAS Protein, N-GST & C-His

References

Datasheet

Human MCIDAS Recombinant Protein (N-GST & C-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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C₁ × V₁ = C₂ × V₂

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Stock Solution

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Human MCIDAS Recombinant Protein (N-GST & C-His) (HA153012)

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