Human MEFV Recombinant Protein (N-His) (HB572012)

Name: Human MEFV Recombinant Protein (N-His)
Brand: abinScience
SKU: HB572012
Price: 211 USD
Availability: InStock

Datasheet Share product Copy info

Price(USD):

Spec:

50ug
100ug
1mg

Number:

Get quote

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.	HB572012
Description	Recombinant Human MEFV Protein, N-His (HB572012) expressed in E. coli, spanning Glu589-Asp781. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	O15553
Protein length	Glu589-Asp781
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	23.48 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Pyrin; Marenostrin; MEFV; MEF; TRIM20
Background	Pyrin is a ~86 kDa protein. Involved in the regulation of innate immunity and the inflammatory response in response to IFNG/IFN-gamma. Organizes autophagic machinery by serving as a platform for the assembly of ULK1, Beclin 1/BECN1, ATG16L1, and ATG8 family members and recognizes specific autophagy targets, thus coordinating target recognition with assembly of the autophagic apparatus and initiation of autophagy. Acts as an autophagy receptor for the degradation of several inflammasome components, including CASP1, NLRP1 and NLRP3, hence preventing excessive IL1B- and IL18-mediated inflammation. However, it can also have a positive effect in the inflammatory pathway, acting as an innate immune sensor that triggers PYCARD/ASC specks formation, caspase-1 activation, and IL1B and IL18 production. Together with AIM2, also acts as a mediator of pyroptosis, necroptosis and apoptosis (PANoptosis), an integral part of host defense against pathogens, in response to bacterial infection. 1. Centola, M. et al. (2000) Blood 95, 3223-31. PMID: 10807793 2. Mansfield, E. et al. (2001) Blood 98, 851-9. PMID: 11468188 3. Yu, JW. et al. (2006) Cell death and differentiation 13, 236-49. PMID: 16037825 4. Chae, JJ. et al. (2006) Proceedings of the National Academy of Sciences of the United States of America 103, 9982-7. PMID: 16785446 5. Papin, S. et al. (2007) Cell death and differentiation 14, 1457-66. PMID: 17431422 6. Yu, JW. et al. (2007) Molecular cell 28, 214-27. PMID: 17964261 7. Chae, JJ. et al. (2008) Blood 112, 1794-803. PMID: 18577712 8. Waite, AL. et al. (2009) Experimental biology and medicine (Maywood, N.J.) 234, 40-52. PMID: 19109554 9. Waite, AL. et al. (2009) PloS one 4, e6147. PMID: 19584923 10. Kimura, T. et al. (2015) The Journal of cell biology 210, 973-89. PMID: 26347139
Note	For research use only

Images

SDS-PAGE

SDS-PAGE for Recombinant Human MEFV Protein, N-His

References

Datasheet

Human MEFV Recombinant Protein (N-His).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Human MEFV Recombinant Protein (N-His) (HB572012)

Overview

Images

References

Datasheet

SDS

Quick access

Contact Us