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Human MET/c-Met/HGFR Recombinant Protein (C-Fc) (HY196011)

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Human MET/c-Met/HGFR Recombinant Protein (C-Fc)

Human MET/c-Met/HGFR Recombinant Protein (C-Fc)

Human MET/c-Met/HGFR Recombinant Protein (C-Fc)

Human MET/c-Met/HGFR Recombinant Protein (C-Fc)

Human MET/c-Met/HGFR Recombinant Protein (C-Fc)

Human MET/c-Met/HGFR Recombinant Protein (C-Fc)

Human MET/c-Met/HGFR Recombinant Protein (C-Fc)

Human MET/c-Met/HGFR Recombinant Protein (C-Fc)

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Overview
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References
Datasheet
SDS

Overview

Catalog No.	HY196011
Description	Recombinant Human MET/c-Met/HGFR Protein, C-Fc (HY196011) expressed in Mammalian Cells, spanning Met1-Thr932. Highlights ●Fc Fusion — Enhanced stability with Protein A/G purification compatibility. ●Mammalian Expression — Proper folding and native glycosylation. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	Mammalian Cells
Accession	P08581
Protein length	Met1-Thr932
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Predicted molecular weight	130.53 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Tyrosine-protein kinase Met, Hepatocyte growth factor receptor, Proto-oncogene c-Met, Scatter factor receptor, HGF receptor, SF receptor, HGF/SF receptor, MET, RCCP2
Background	Hepatocyte growth factor receptor (MET/HGFR) is a ~155 kDa protein. Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to hepatocyte growth factor/HGF ligand. Regulates many physiological processes including proliferation, scattering, morphogenesis and survival. Ligand binding at the cell surface induces autophosphorylation of MET on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1. Recruitment of these downstream effectors by MET leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. MET is the therapeutic target of amivantamab (Rybrevant).
Note	For research use only.

Images

SDS-PAGE

SDS-PAGE for Recombinant Human MET/c-Met/HGFR Protein, C-Fc
Bioactivity

Detects Emibetuzumab in indirect ELISAs.
Bioactivity

Detects Telisotuzumab in indirect ELISAs.
Western blot

Detected by Anti-MET/c-Met/HGFR monoclonal antibody (Catalog No: HY196016)

References

HY196011-SDS-PAGE-1.jpg

Datasheet

Human MET/c-Met/HGFR Recombinant Protein (C-Fc).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Formula

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Stock Solution

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Working Solution

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Human MET/c-Met/HGFR Recombinant Protein (C-Fc)