Human NAP1L1 Recombinant Protein (N-His) (HX949012)

Name: Human NAP1L1 Recombinant Protein (N-His)
Brand: abinScience
SKU: HX949012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HX949012
Description	Recombinant Human NAP1L1 Protein, N-His (HX949012) expressed in E. coli, spanning Arg72-Glu356. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P55209
Protein length	Arg72-Glu356
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	35.65 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	NAP-1-related protein, NRP, hNRP, Nucleosome assembly protein 1-like 1, NAP1L1
Background	Nucleosome assembly protein 1-like 1 (NAP1L1) is a ~45 kDa protein. Histone chaperone that plays a role in the nuclear import of H2A-H2B and nucleosome assembly. Also participates in several important DNA repair mechanisms: greatly enhances ERCC6-mediated chromatin remodeling which is essential for transcription-coupled nucleotide excision DNA repair. Also stimulates homologous recombination (HR) by RAD51 and RAD54 which is essential in mitotic DNA double strand break (DSB) repair. Plays a key role in the regulation of embryonic neurogenesis. Promotes the proliferation of neural progenitors and inhibits neuronal differentiation during cortical development. 1. Okuwaki, M. et al. (2010) Genes to cells : devoted to molecular & cellular mechanisms 15, 13-27. PMID: 20002496 2. Mehrotra, PV. et al. (2011) Molecular cell 41, 46-55. PMID: 21211722 3. Ohtomo, H. et al. (2016) Genes to cells : devoted to molecular & cellular mechanisms 21, 252-63. PMID: 26841755 4. Lee, JY. et al. (2017) Nucleic acids research 45, 4696-4707. PMID: 28369616 5. Machida, S. et al. (2014) Scientific reports 4, 4863. PMID: 24798879
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human NAP1L1 Protein, N-His

References

HX949012-SDS-PAGE-1.jpg

Datasheet

Human NAP1L1 Recombinant Protein (N-His).pdf

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Formula

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Human NAP1L1 Recombinant Protein (N-His) (HX949012)

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