Human NPC1 Recombinant Protein (C-Fc) (HT011021)

Name: Human NPC1 Recombinant Protein (C-Fc)
Brand: abinScience
SKU: HT011021
Price: 298 USD
Availability: InStock

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Overview

Catalog No.	HT011021
Description	Recombinant Human NPC1 Protein, C-Fc (HT011021) expressed in Mammalian Cells, spanning Arg372-Phe622. Purity: >90% by SDS-PAGE. Highlights ●Fc Fusion — Enhanced stability with Protein A/G purification compatibility. ●Mammalian Expression — Proper folding and native glycosylation. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	Mammalian cells
Accession	O15118
Protein length	Arg372-Phe622
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	57.05 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	NPC intracellular cholesterol transporter 1, NPC1, Niemann-Pick C1 protein
Background	NPC intracellular cholesterol transporter 1 is a ~142 kDa protein. Intracellular cholesterol transporter which acts in concert with NPC2 and plays an important role in the egress of cholesterol from the endosomal/lysosomal compartment. Unesterified cholesterol that has been released from LDLs in the lumen of the late endosomes/lysosomes is transferred by NPC2 to the cholesterol-binding pocket in the N-terminal domain of NPC1. Cholesterol binds to NPC1 with the hydroxyl group buried in the binding pocket. Binds oxysterol with higher affinity than cholesterol. May play a role in vesicular trafficking in glia, a process that may be crucial for maintaining the structural and functional integrity of nerve terminals (Probable). 1. Davies, JP. et al. (2000) The Journal of biological chemistry 275, 24367-74. PMID: 10821832 2. Blom, TS. et al. (2003) Human molecular genetics 12, 257-72. PMID: 12554680 3. Infante, RE. et al. (2008) Proceedings of the National Academy of Sciences of the United States of America 105, 15287-92. PMID: 18772377 4. Gong, X. et al. (2016) Cell 165, 1467-1478. PMID: 27238017 5. Carstea, ED. et al. (1997) Science (New York, N.Y.) 277, 228-31. PMID: 9211849 6. Watari, H. et al. (1999) Proceedings of the National Academy of Sciences of the United States of America 96, 805-10. PMID: 9927649 7. Kwon, HJ. et al. (2009) Cell 137, 1213-24. PMID: 19563754 8. Ebrahimi-Fakhari, D. et al. (2016) Human molecular genetics 25, 3588-3599. PMID: 27378690
Note	For research use only

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Datasheet

Human NPC1 Recombinant Protein (C-Fc).pdf

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Human NPC1 Recombinant Protein (C-Fc) (HT011021)

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