Human PTK7/CCK-4 Recombinant Protein (C-His) (HC437011)

Name: Human PTK7/CCK-4 Recombinant Protein (C-His)
Brand: abinScience
SKU: HC437011
Price: 298 USD
Availability: InStock

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Overview

Catalog No.	HC437011
Description	Recombinant Human PTK7/CCK-4 Protein, C-His (HC437011) expressed in Mammalian Cells, spanning Met1-Thr704. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●Mammalian Expression — Proper folding and native glycosylation. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	Mammalian cells
Accession	Q13308
Protein length	Met1-Thr704
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	78.68 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Protein-tyrosine kinase 7, Inactive tyrosine-protein kinase 7, CCK4, PTK7, Colon carcinoma kinase 4, Tyrosine-protein kinase-like 7, CCK-4, Pseudo tyrosine kinase receptor 7
Background	Inactive tyrosine-protein kinase 7 (PTK7/CCK-4) is a ~118 kDa protein. Inactive tyrosine kinase involved in Wnt signaling pathway. Component of both the non-canonical (also known as the Wnt/planar cell polarity signaling) and the canonical Wnt signaling pathway. Functions in cell adhesion, cell migration, cell polarity, proliferation, actin cytoskeleton reorganization and apoptosis. Has a role in embryogenesis, epithelial tissue organization and angiogenesis.
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human PTK7/CCK-4 Protein, C-His
Bioactivity

Detects Cofetuzumab in indirect ELISAs.
Bioactivity

Detected by SAA2288 (Catalog No: HC437013) in indirect ELISA.

References

Datasheet

Human PTK7/CCK-4 Recombinant Protein (C-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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Human PTK7/CCK-4 Recombinant Protein (C-His) (HC437011)

Overview

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References

Datasheet

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