Human RB1CC1 Recombinant Protein (N-His) (HP312012)

Name: Human RB1CC1 Recombinant Protein (N-His)
Brand: abinScience
SKU: HP312012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HP312012
Description	Recombinant Human RB1CC1 Protein, N-His (HP312012) expressed in E. coli, spanning Glu1447-Val1594. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q8TDY2
Protein length	Glu1447-Val1594
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	19.48 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	FAK family kinase-interacting protein of 200 kDa, RB1CC1, FIP200, RBICC, RB1-inducible coiled-coil protein 1, KIAA0203
Background	RB1-inducible coiled-coil protein 1 (RB1CC1) is a ~183 kDa protein. Involved in autophagy. Regulates early events but also late events of autophagosome formation through direct interaction with Atg16L1. Required for the formation of the autophagosome-like double-membrane structure that surrounds the Salmonella-containing vacuole (SCV) during S.typhimurium infection and subsequent xenophagy. Involved in repair of DNA damage caused by ionizing radiation, which subsequently improves cell survival by decreasing apoptosis. Inhibits PTK2/FAK1 and PTK2B/PYK2 kinase activity, affecting their downstream signaling pathways. 1. Morselli, E. et al. (2011) Cell cycle (Georgetown, Tex.) 10, 2763-9. PMID: 21775823 2. Nishimura, T. et al. (2013) EMBO reports 14, 284-91. PMID: 23392225 3. Ueda, H. et al. (2000) The Journal of cell biology 149, 423-30. PMID: 10769033 4. Abbi, S. et al. (2002) Molecular biology of the cell 13, 3178-91. PMID: 12221124 5. Chano, T. et al. (2002) Gene 291, 29-34. PMID: 12095676 6. Kontani, K. et al. (2003) International journal of molecular medicine 12, 767-9. PMID: 14533007 7. Chano, T. et al. (2002) The American journal of pathology 161, 359-64. PMID: 12163359
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human RB1CC1 Protein, N-His

References

HP312012-SDS-PAGE-1.jpg

Datasheet

Human RB1CC1 Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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C₁ × V₁ = C₂ × V₂

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Human RB1CC1 Recombinant Protein (N-His) (HP312012)

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