ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Homo sapiens (Human)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Predicted molecular weight
28.81 kDa
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Sentrin-specific protease 1 (SENP1) is a ~73 kDa protein. Protease that catalyzes two essential functions in the SUMO pathway. The first is the hydrolysis of an alpha-linked peptide bond at the C-terminal end of the small ubiquitin-like modifier (SUMO) propeptides, SUMO1, SUMO2 and SUMO3 leading to the mature form of the proteins. The second is the deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins, by cleaving an epsilon-linked peptide bond between the C-terminal glycine of the mature SUMO and the lysine epsilon-amino group of the target protein. Deconjugates SUMO1 from HIPK2. Deconjugates SUMO1 from HDAC1 and BHLHE40/DEC1, which decreases its transcriptional repression activity.
1. Gong, L. et al. (2000) The Journal of biological chemistry 275, 3355-9. PMID: 10652325
2. Cheng, J. et al. (2004) Molecular and cellular biology 24, 6021-8. PMID: 15199155
3. Xu, Z. et al. (2005) The Biochemical journal 386, 325-30. PMID: 15487983
4. Kim, YH. et al. (2005) FEBS letters 579, 6272-8. PMID: 16253240
5. Shen, LN. et al. (2006) The Biochemical journal 397, 279-88. PMID: 16553580
6. Hong, Y. et al. (2011) PloS one 6, e23046. PMID: 21829689
7. Cong, L. et al. (2011) The Journal of biological chemistry 286, 43793-43808. PMID: 21965678
8. Li, S. et al. (2013) Oncogene 32, 4883-91. PMID: 23160374
9. Bi, H. et al. (2014) Molecular biology of the cell 25, 2499-508. PMID: 24943844
10. Park, JH. et al. (2014) Nature communications 5, 5483. PMID: 25406032
Note
For research use only.
Images
SDS-PAGE
SDS-PAGE for Recombinant Human SENP1 Protein, N-His
中文
English
한국어
日本語
Español
Français
Русский
