Human SLC15A1 Recombinant Protein (N-His) (HA507012)

Name: Human SLC15A1 Recombinant Protein (N-His)
Brand: abinScience
SKU: HA507012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HA507012
Description	Recombinant Human SLC15A1 Protein, N-His (HA507012) expressed in E. coli, spanning Asp383-Gln584. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P46059
Protein length	Asp383-Gln584
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	24.91 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Solute carrier family 15 member 1, Intestinal H(+)/peptide cotransporter, Oligopeptide transporter, small intestine isoform, Peptide transporter 1, SLC15A1, PEPT1
Background	Solute carrier family 15 member 1 is a ~78 kDa protein. Electrogenic proton-coupled amino-acid transporter that transports oligopeptides of 2 to 4 amino acids with a preference for dipeptides. Transports neutral and monovalently charged peptides with a proton to peptide stoichiometry of 1:1 or 2:1. Primarily responsible for the absorption of dietary di- and tripeptides from the small intestinal lumen. Mediates transepithelial transport of muramyl and N-formylated bacterial dipeptides contributing to recognition of pathogenic bacteria by the mucosal immune system. 1. Vavricka, SR. et al. (2004) Gastroenterology 127, 1401-9. PMID: 15521010 2. Sala-Rabanal, M. et al. (2008) American journal of physiology. Renal physiology 294, F1422-32. PMID: 18367661 3. Xu, L. et al. (2009) Pharmaceutical research 26, 2358-66. PMID: 19685173 4. Beale, JH. et al. (2015) Structure (London, England : 1993) 23, 1889-1899. PMID: 26320580 5. Liang, R. et al. (1995) The Journal of biological chemistry 270, 6456-63. PMID: 7896779 6. Mackenzie, B. et al. (1996) Biochimica et biophysica acta 1284, 125-8. PMID: 8914574 7. Merlin, D. et al. (1998) The Journal of clinical investigation 102, 2011-8. PMID: 9835627
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human SLC15A1 Protein, N-His

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Datasheet

Human SLC15A1 Recombinant Protein (N-His).pdf

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Human SLC15A1 Recombinant Protein (N-His) (HA507012)

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