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Human SLC22A5 Recombinant Protein (N-GST & C-His) (HF955022)

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Overview
Catalog No.HF955022
Description
Recombinant Human SLC22A5 Protein, N-GST & C-His (HF955022) expressed in E. coli, spanning Thr45-Lys141. Purity: >90% by SDS-PAGE.
Highlights
  • His-Tagged — N-terminal 6×His tag for IMAC purification.
  • E. coli Expression — High-yield, cost-effective production.
  • High Purity — >90% purity verified by SDS-PAGE.
Expression systemE. coli
AccessionO76082
Protein lengthThr45-Lys141
ApplicationsELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
SpeciesHomo sapiens (Human)
Nature Recombinant
Endotoxin level Please contact with the lab for this information.
Purity >90% as determined by SDS-PAGE.
Predicted molecular weight 39.35 kDa
Form Lyophilized
Storage buffer Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate NamesHigh-affinity sodium-dependent carnitine cotransporter, OCTN2, Organic cation/carnitine transporter 2, SLC22A5, Solute carrier family 22 member 5
Background

Organic cation/carnitine transporter 2 is a ~62 kDa protein. Sodium-ion dependent, high affinity carnitine transporter. Involved in the active cellular uptake of carnitine. Transports one sodium ion with one molecule of carnitine. Also transports organic cations such as tetraethylammonium (TEA) without the involvement of sodium. Relative uptake activity ratio of carnitine to TEA is 11.3.

1. Wu, X. et al. (1999) The Journal of pharmacology and experimental therapeutics 290, 1482-92. PMID: 10454528
2. Ohashi, R. et al. (1999) The Journal of pharmacology and experimental therapeutics 291, 778-84. PMID: 10525100
3. Wagner, CA. et al. (2000) American journal of physiology. Renal physiology 279, F584-91. PMID: 10966938
4. Maekawa, S. et al. (2007) Biochimica et biophysica acta 1773, 1000-6. PMID: 17509700
5. Fujiya, M. et al. (2011) Inflammatory bowel diseases 17, 907-16. PMID: 20722056
6. Yee, SW. et al. (2020) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 34, 15734-15752. PMID: 33124720
7. Fujiya, M. et al. (2007) Cell host & microbe 1, 299-308. PMID: 18005709
Note For research use only
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Formula
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