Human SLC25A1 Recombinant Protein (N-GST & C-His) (HC027012)

Name: Human SLC25A1 Recombinant Protein (N-GST & C-His)
Brand: abinScience
SKU: HC027012
Price: 211 USD
Availability: InStock

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Catalog No.	HC027012
Description	Recombinant Human SLC25A1 Protein, N-GST & C-His (HC027012) expressed in E. coli. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P53007
Protein length	Glu47-Arg85&Glu106-Gly128&Glu144-Gln182&Leu241-Gly278
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	44.78 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Tricarboxylate transport protein, mitochondrial; Citrate transport protein; CTP; Mitochondrial citrate carrier; CIC; Solute carrier family 25 member 1; Tricarboxylate carrier protein; SLC25A1; SLC20A3
Background	Tricarboxylate transport protein, mitochondrial is a ~34 kDa protein. Mitochondrial electroneutral antiporter that exports citrate from the mitochondria into the cytosol in exchange for malate. Also able to mediate the exchange of citrate for isocitrate, phosphoenolpyruvate, cis-aconitate and to a lesser extent trans-aconitate, maleate and succinate. Substrate exchange across the membrane occurs consecutively with one substrate being transported first, then dissociating from the substrate binding site before the second substrate binds for transport in the opposite direction. In the cytoplasm, citrate plays important roles in fatty acid and sterol synthesis, regulation of glycolysis, protein acetylation, and other physiopathological processes. 1. Chaouch, A. et al. (2014) Journal of neuromuscular diseases 1, 75-90. PMID: 26870663 2. Majd, H. et al. (2018) Biochimica et biophysica acta. Bioenergetics 1859, 1-7. PMID: 29031613 3. Pop, A. et al. (2018) Journal of inherited metabolic disease 41, 169-180. PMID: 29238895 4. Li, W. et al. (2025) The EMBO journal 44, 1641-1662. PMID: 39881208 5. Cimadamore-Werthein, C. et al. (2024) The EMBO journal 43, 3450-3465. PMID: 38937634
Note	For research use only

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SDS-PAGE

SDS-PAGE for Recombinant Human SLC25A1 Protein, N-GST & C-His

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Datasheet

Human SLC25A1 Recombinant Protein (N-GST & C-His).pdf

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Human SLC25A1 Recombinant Protein (N-GST & C-His) (HC027012)

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