ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Homo sapiens (Human)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Predicted molecular weight
36.35 kDa
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Equilibrative nucleoside transporter 2 is a ~50 kDa protein. Bidirectional uniporter involved in the facilitative transport of nucleosides and nucleobases, and contributes to maintaining their cellular homeostasis. Functions as a Na(+)-independent, passive transporter. Involved in the transport of nucleosides such as inosine, adenosine, uridine, thymidine, cytidine and guanosine. Also able to transport purine nucleobases (hypoxanthine, adenine, guanine) and pyrimidine nucleobases (thymine, uracil). Involved in nucleoside transport at basolateral membrane of kidney cells, allowing liver absorption of nucleoside metabolites.
1. Ward, JL. et al. (2000) The Journal of biological chemistry 275, 8375-81. PMID: 10722669
2. Mangravite, LM. et al. (2003) American journal of physiology. Renal physiology 284, F902-10. PMID: 12527552
3. Ward, JL. et al. (2003) Archives of biochemistry and biophysics 411, 19-26. PMID: 12590919
4. Owen, RP. et al. (2006) Drug metabolism and disposition: the biological fate of chemicals 34, 12-5. PMID: 16214850
5. Yao, SY. et al. (2011) The Journal of biological chemistry 286, 32552-62. PMID: 21795683
6. Griffiths, M. et al. (1997) The Biochemical journal 328 ( Pt 3), 739-43. PMID: 9396714
7. Crawford, CR. et al. (1998) The Journal of biological chemistry 273, 5288-93. PMID: 9478986
中文
English
한국어
日本語
Español
Français
Русский
