Human SLC6A4 Recombinant Protein (N-GST) (HB725012)

Name: Human SLC6A4 Recombinant Protein (N-GST)
Brand: abinScience
SKU: HB725012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HB725012
Description	Recombinant Human SLC6A4 Protein, N-GST (HB725012) expressed in E. coli, spanning Ala181-Ser252. Purity: >90% by SDS-PAGE. Highlights ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P31645
Protein length	Ala181-Ser252
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	35.13 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	5HT transporter, 5HTT, SERT, Sodium-dependent serotonin transporter, Solute carrier family 6 member 4, HTT, SLC6A4
Background	Sodium-dependent serotonin transporter (SLC6A4/SERT) is a ~70 kDa protein. Serotonin transporter that cotransports serotonin with one Na(+) ion in exchange for one K(+) ion and possibly one proton in an overall electroneutral transport cycle. Transports serotonin across the plasma membrane from the extracellular compartment to the cytosol thus limiting serotonin intercellular signaling. Essential for serotonin homeostasis in the central nervous system. In the developing somatosensory cortex, acts in glutamatergic neurons to control serotonin uptake and its trophic functions accounting for proper spatial organization of cortical neurons and elaboration of sensory circuits. In the mature cortex, acts primarily in brainstem raphe neurons to mediate serotonin uptake from the synaptic cleft back into the pre-synaptic terminal thus terminating serotonin signaling at the synapse. 1. Mortensen, OV. et al. (1999) Brain research. Molecular brain research 71, 120-6. PMID: 10407194 2. Kilic, F. et al. (2003) Molecular pharmacology 64, 440-6. PMID: 12869649 3. Henry, LK. et al. (2011) The Journal of biological chemistry 286, 30823-30836. PMID: 21730057 4. Coleman, JA. et al. (2016) Nature 532, 334-9. PMID: 27049939 5. Hasenhuetl, PS. et al. (2016) The Journal of biological chemistry 291, 25864-25876. PMID: 27756841 6. Yang, D. et al. (2021) Science advances 7, eabl3857. PMID: 34851672 7. Brenner, B. et al. (2007) Journal of neurochemistry 102, 206-15. PMID: 17506858 8. Carneiro, AM. et al. (2008) The Journal of clinical investigation 118, 1544-52. PMID: 18317590
Note	For research use only.