Human SPHK2 Recombinant Protein (N-His) (HP741012)

Name: Human SPHK2 Recombinant Protein (N-His)
Brand: abinScience
SKU: HP741012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HP741012
Description	Recombinant Human SPHK2 Protein, N-His (HP741012) expressed in E. coli, spanning Gly170-His370. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q9NRA0
Protein length	Gly170-His370
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	23.80 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	SK2, Sphingosine kinase 2, SPHK2, SK 2, SPK 2
Background	Sphingosine kinase 2 (SPHK2) is a ~69 kDa protein. Catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-dihydrosphingosine, D-erythro-sphingosine and L-threo-dihydrosphingosine. Binds phosphoinositides. In contrast to prosurvival SPHK1, has a positive effect on intracellular ceramide levels, inhibits cells growth and enhances apoptosis. In mitochondria, is important for cytochrome-c oxidase assembly and mitochondrial respiration. 1. Igarashi, N. et al. (2003) The Journal of biological chemistry 278, 46832-9. PMID: 12954646 2. Don, AS. et al. (2009) Biochemical and biophysical research communications 380, 87-92. PMID: 19168031 3. Maceyka, M. et al. (2005) The Journal of biological chemistry 280, 37118-29. PMID: 16118219 4. Strub, GM. et al. (2011) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 25, 600-12. PMID: 20959514 5. Hait, NC. et al. (2009) Science (New York, N.Y.) 325, 1254-7. PMID: 19729656 6. Okada, T. et al. (2005) The Journal of biological chemistry 280, 36318-25. PMID: 16103110
Note	For research use only.

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SDS-PAGE for Human SPHK2 Recombinant Protein (N-His)

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Datasheet

Human SPHK2 Recombinant Protein (N-His).pdf

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Formula

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Human SPHK2 Recombinant Protein (N-His) (HP741012)

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