Human SRP68 Recombinant Protein (N-His) (HP400012)

Name: Human SRP68 Recombinant Protein (N-His)
Brand: abinScience
SKU: HP400012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HP400012
Description	Recombinant Human SRP68 Protein, N-His (HP400012) expressed in E. coli, spanning Met293-Gln540. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q9UHB9
Protein length	Met293-Gln540
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	30.65 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Signal recognition particle 68 kDa protein, Signal recognition particle subunit SRP68, SRP68
Background	Signal recognition particle subunit SRP68 is a ~70 kDa protein. Component of the signal recognition particle (SRP) complex, a ribonucleoprotein complex that mediates the cotranslational targeting of secretory and membrane proteins to the endoplasmic reticulum (ER). The SRP complex interacts with the signal sequence in nascent secretory and membrane proteins and directs them to the membrane of the ER. The SRP complex targets the ribosome-nascent chain complex to the SRP receptor (SR), which is anchored in the ER, where SR compaction and GTPase rearrangement drive cotranslational protein translocation into the ER. Binds the signal recognition particle RNA (7SL RNA), SRP72 binds to this complex subsequently. The SRP complex possibly participates in the elongation arrest function. 1. Lee, JH. et al. (2021) Science advances 7. PMID: 34020957 2. Iakhiaeva, E. et al. (2006) Protein science : a publication of the Protein Society 15, 1290-302. PMID: 16672232 3. Becker, MM. et al. (2017) Nucleic acids research 45, 470-481. PMID: 27899666
Note	For research use only.

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SDS-PAGE for Recombinant Human SRP68 Protein, N-His

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Datasheet

Human SRP68 Recombinant Protein (N-His).pdf

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Human SRP68 Recombinant Protein (N-His) (HP400012)

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