Human SSNA1 Recombinant Protein (N-His-SUMO) (HT479012)

Name: Human SSNA1 Recombinant Protein (N-His-SUMO)
Brand: abinScience
SKU: HT479012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HT479012
Description	Recombinant Human SSNA1 Protein, N-His-SUMO (HT479012) expressed in E. coli, spanning Leu15-Arg98. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	O43805
Protein length	Leu15-Arg98
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	22.30 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	SSNA1, NA14, Sjoegren syndrome nuclear autoantigen 1, Nuclear autoantigen of 14 kDa
Background	Microtubule nucleation factor SSNA1 is a ~13 kDa protein. Microtubule-binding protein which stabilizes dynamic microtubules by slowing growth and shrinkage at both plus and minus ends and serves as a sensor of microtubule damage, protecting microtubules from the microtubule-severing enzyme SPAST. Induces microtubule branching which is mediated by the formation of long SSNA1 fibrils which guide microtubule protofilaments to split apart from the mother microtubule and form daughter microtubules. Plays a role in axon outgrowth and branching. Required for cell division. 1. Lawrence, EJ. et al. (2021) eLife 10. PMID: 34970964 2. Goyal, U. et al. (2014) PloS one 9, e112428. PMID: 25390646
Note	For research use only.

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SDS-PAGE for Recombinant Human SSNA1 Protein, N-His-SUMO

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Datasheet

Human SSNA1 Recombinant Protein (N-His-SUMO).pdf

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Human SSNA1 Recombinant Protein (N-His-SUMO) (HT479012)

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