Human THBS1 Recombinant Protein (N-His) (HY205012)

Name: Human THBS1 Recombinant Protein (N-His)
Brand: abinScience
SKU: HY205012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HY205012
Description	Recombinant Human THBS1 Protein, N-His (HY205012) expressed in E. coli, spanning Ser24-Thr221. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P07996
Protein length	Ser24-Thr221
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	23.91 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	TSP, THBS1, TSP1, Thrombospondin-1, Glycoprotein G
Background	Thrombospondin-1 (THBS1) is a ~129 kDa protein. Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Multifunctional, involved in inflammation, angiogenesis, wound healing, reactive oxygen species (ROS) signaling, nitrous oxide (NO) signaling, apoptosis, senescence, aging, cellular self-renewal, stemness, and cardiovascular and metabolic homeostasis. Negatively modulates dendritic cell activation and cytokine release, as part of an autocrine feedback loop, contributing to the resolution of inflammation and immune homeostasis. Ligand for receptor CD47. Modulates nitrous oxide (NO) signaling via CD47, hence playing a role as a pressor agent, supporting blood pressure. 1. Kvansakul, M. et al. (2004) The EMBO journal 23, 1223-33. PMID: 15014436 2. Adams, JC. et al. (2008) Journal of cell science 121, 784-95. PMID: 18285447 3. Lawler, J. et al. (1986) The Journal of cell biology 103, 1635-48. PMID: 2430973 4. Lahav, J. et al. (1984) European journal of biochemistry 145, 151-6. PMID: 6489349 5. Jiménez, B. et al. (2000) Nature medicine 6, 41-8. PMID: 10613822 6. Simantov, R. et al. (2001) The Journal of clinical investigation 107, 45-52. PMID: 11134179 7. Asch, AS. et al. (1992) Biochemical and biophysical research communications 182, 1208-17. PMID: 1371676 8. Doyen, V. et al. (2003) The Journal of experimental medicine 198, 1277-83. PMID: 14568985 9. Yao, M. et al. (2014) Journal of the American Society of Nephrology : JASN 25, 1171-86. PMID: 24511121 10. Meijles, DN. et al. (2017) Science signaling 10. PMID: 29042481
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human THBS1 Protein, N-His

References

HY205012-SDS-PAGE-1.jpg
Multi-omics analysis reveals the interaction between the complement system and the coagulation cascade in the development of endometriosis

Datasheet

Human THBS1 Recombinant Protein (N-His).pdf

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Human THBS1 Recombinant Protein (N-His) (HY205012)

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