Human TRIM37 Recombinant Protein (N-His) (HF616012)

Name: Human TRIM37 Recombinant Protein (N-His)
Brand: abinScience
SKU: HF616012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HF616012
Description	Recombinant Human TRIM37 Protein, N-His (HF616012) expressed in E. coli, spanning Ser275-Ser405. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	O94972
Protein length	Ser275-Ser405
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	17.44 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	E3 ubiquitin-protein ligase TRIM37, 2.3.2.27, Mulibrey nanism protein, RING-type E3 ubiquitin transferase TRIM37, Tripartite motif-containing protein 37, TRIM37, KIAA0898, MUL, POB1
Background	E3 ubiquitin-protein ligase TRIM37 is a ~107 kDa protein. E3 ubiquitin-protein ligase required to prevent centriole reduplication. Probably acts by ubiquitinating positive regulators of centriole reduplication. Mediates monoubiquitination of 'Lys-119' of histone H2A (H2AK119Ub), a specific tag for epigenetic transcriptional repression: associates with some Polycomb group (PcG) multiprotein PRC2-like complex and mediates repression of target genes. Also acts as a positive regulator of peroxisome import by mediating monoubiquitination of PEX5 at 'Lys-472': monoubiquitination promotes PEX5 stabilitation by preventing its polyubiquitination and degradation by the proteasome. Has anti-HIV activity. 1. Kallijärvi, J. et al. (2005) Experimental cell research 308, 146-55. PMID: 15885686 2. Balestra, FR. et al. (2013) Developmental cell 25, 555-71. PMID: 23769972 3. Bhatnagar, S. et al. (2014) Nature 516, 116-20. PMID: 25470042 4. Wang, W. et al. (2017) The Journal of cell biology 216, 2843-2858. PMID: 28724525 5. Tabah, AA. et al. (2014) The Journal of general virology 95, 960-967. PMID: 24317724
Note	For research use only.

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SDS-PAGE for Recombinant Human TRIM37 Protein, N-His

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Datasheet

Human TRIM37 Recombinant Protein (N-His).pdf

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Human TRIM37 Recombinant Protein (N-His) (HF616012)

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