Human TSPAN1 Recombinant Protein (N-His) (HT497012)

Name: Human TSPAN1 Recombinant Protein (N-His)
Brand: abinScience
SKU: HT497012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HT497012
Description	Recombinant Human TSPAN1 Protein, N-His (HT497012) expressed in E. coli, spanning Tyr110-Asn211. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	O60635
Protein length	Tyr110-Asn211
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	14.03 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Tetraspanin-1, Tspan-1, Tetraspan NET-1, Tetraspanin TM4-C, TSPAN1
Background	Tetraspanin-1 (TSPAN1) is a ~26 kDa protein. Structural component of specialized membrane microdomains known as tetraspanin-enriched microdomains (TERMs), which act as platforms for receptor clustering and signaling. Participates thereby in diverse biological functions such as cell signal transduction, adhesion, migration and protein trafficking. Regulates neuronal differentiation in response to NGF by facilitating NGF-mediated activation of NTRK1/TRKA receptor tyrosine kinase and subsequent downstream signaling pathways. Plays a role in the inhibition of TNFalpha-induced apoptosis. Mechanistically, inhibits the NF-kappa-B signaling pathway by blocking phosphorylation of CHUK. 1. Tian, J. et al. (2018) Molecular medicine reports 18, 3280-3288. PMID: 30066932 2. Yang, L. et al. (2018) Inflammation research : official journal of the European Histamine Research Society ... [et al.] 67, 951-964. PMID: 30291375 3. Nabokina, SM. et al. (2011) American journal of physiology. Gastrointestinal and liver physiology 301, G808-13. PMID: 21836059
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human TSPAN1 Protein, N-His

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Datasheet

Human TSPAN1 Recombinant Protein (N-His).pdf

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Human TSPAN1 Recombinant Protein (N-His) (HT497012)

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