ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Homo sapiens (Human)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Predicted molecular weight
8.82 kDa
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Ubiquitin-like modifier-activating enzyme 7 (UBA7) is a ~111 kDa protein. E1-activating enzyme that catalyzes the covalent conjugation of the ubiquitin-like protein product of ISG15 to additional interferon stimulated proteins (ISGs) as well as other cellular proteins such as P53 in a process termed protein ISGylation. Plays an essential role in antiviral immunity together with ISG15 by restricting the replication of many viruses including rabies virus, influenza virus, sindbis virus, rotavirus or human cytomegalovirus. For example, ISG15 modification of influenza A protein NS1 disrupts the association of the NS1 with importin-alpha leading to NS1 nuclear import inhibition. ISGylation of human cytomegalovirs protein UL26 regulates its stability and inhibits its activities to suppress NF-kappa-B signaling.
1. Park, JH. et al. (2016) Nature communications 7, 12513. PMID: 27545325
2. Lenschow, DJ. et al. (2005) Journal of virology 79, 13974-83. PMID: 16254333
3. Giannakopoulos, NV. et al. (2009) Journal of virology 83, 1602-10. PMID: 19073728
4. Zhao, P. et al. (2017) Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases 56, 44-53. PMID: 29056542
5. Lee, MK. et al. (2018) Journal of virology 92. PMID: 29743376
6. Sarkar, R. et al. (2023) Cellular signalling 112, 110891. PMID: 37722521
7. Zhao, C. et al. (2010) Proceedings of the National Academy of Sciences of the United States of America 107, 2253-8. PMID: 20133869
8. Kim, YJ. et al. (2016) PLoS pathogens 12, e1005850. PMID: 27564865
Note
For research use only.
Images
SDS-PAGE
SDS-PAGE for Recombinant Human UBA7 Protein, N-His
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