

| Catalog No. | HD110012 |
|---|---|
| Description |
Recombinant Human UBE2G2 Protein, N-His (HD110012) expressed in E. coli, spanning Met1-Leu165. Purity: >90% by SDS-PAGE.
Highlights
|
| Expression system | E. coli |
| Accession | P60604 |
| Protein length | Met1-Leu165 |
| Applications | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| Species | Homo sapiens (Human) |
| Nature | Recombinant |
| Endotoxin level | Please contact with the lab for this information. |
| Purity | >90% as determined by SDS-PAGE. |
| Predicted molecular weight | 20.73 kDa |
| Form | Lyophilized |
| Storage buffer | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| Reconstitution | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Shipping | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Alternate Names | E2 ubiquitin-conjugating enzyme G2, EC:2.3.2.23, UBC7, UBE2G2, Ubiquitin carrier protein G2, Ubiquitin-conjugating enzyme E2 G2, Ubiquitin-protein ligase G2 |
| Background | Ubiquitin-conjugating enzyme E2 G2 is a ~18 kDa protein. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Involved in endoplasmic reticulum-associated degradation (ERAD). Required for sterol-induced ubiquitination of 3-hydroxy-3-methylglutaryl coenzyme A reductase and its subsequent proteasomal degradation. 1. David, Y. et al. (2010) The Journal of biological chemistry 285, 8595-604. PMID: 20061386 2. Sato, T. et al. (2012) Molecular cell 47, 99-110. PMID: 22607976 3. Jo, Y. et al. (2013) Molecular biology of the cell 24, 169-83. PMID: 23223569 |
| Note | For research use only |
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