ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Homo sapiens (Human)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Predicted molecular weight
20.80 kDa
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
UDP-glucuronosyltransferase 2B7 (UGT2B7) is a ~60 kDa protein. UDP-glucuronosyltransferase (UGT) that catalyzes phase II biotransformation reactions in which lipophilic substrates are conjugated with glucuronic acid to increase the metabolite's water solubility, thereby facilitating excretion into either the urine or bile. Essential for the elimination and detoxification of drugs, xenobiotics and endogenous compounds. Catalyzes the glucuronidation of endogenous steroid hormones such as androgens (epitestosterone, androsterone) and estrogens (estradiol, epiestradiol, estriol, catechol estrogens). Also regulates the levels of retinoic acid, a major metabolite of vitamin A involved in apoptosis, cellular growth and differentiation, and embryonic development. Contributes to bile acid (BA) detoxification by catalyzing the glucuronidation of BA substrates, which are natural detergents for dietary lipids absorption.
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2. Picard, N. et al. (2005) Drug metabolism and disposition: the biological fate of chemicals 33, 139-46. PMID: 15470161
3. Lépine, J. et al. (2004) The Journal of clinical endocrinology and metabolism 89, 5222-32. PMID: 15472229
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6. Itäaho, K. et al. (2008) Drug metabolism and disposition: the biological fate of chemicals 36, 2307-15. PMID: 18719240
7. Sten, T. et al. (2009) Drug metabolism and disposition: the biological fate of chemicals 37, 417-23. PMID: 19022937
8. Sneitz, N. et al. (2013) Drug metabolism and disposition: the biological fate of chemicals 41, 582-91. PMID: 23288867
9. Perreault, M. et al. (2013) Drug metabolism and disposition: the biological fate of chemicals 41, 1616-20. PMID: 23756265
10. Kallionpää, RA. et al. (2015) The Journal of steroid biochemistry and molecular biology 154, 104-11. PMID: 26220143
Note
For research use only.
Images
SDS-PAGE
SDS-PAGE for Recombinant Human UGT2B7 Protein, N-His
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