Human YTHDF3 Recombinant Protein (N-His) (HA146012)

Name: Human YTHDF3 Recombinant Protein (N-His)
Brand: abinScience
SKU: HA146012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HA146012
Description	Recombinant Human YTHDF3 Protein, N-His (HA146012) expressed in E. coli, spanning Gly416-Glu572. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q7Z739
Protein length	Gly416-Glu572
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	20.52 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	YTH domain-containing family protein 3, DF3, YTHDF3
Background	YTH domain-containing family protein 3 (YTHDF3) is a ~63 kDa protein. Specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs, and regulates their stability. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in mRNA stability and processing. Acts as a regulator of mRNA stability by promoting degradation of m6A-containing mRNAs via interaction with the CCR4-NOT complex or PAN3. The YTHDF paralogs (YTHDF1, YTHDF2 and YTHDF3) share m6A-containing mRNAs targets and act redundantly to mediate mRNA degradation and cellular differentiation. Acts as a negative regulator of type I interferon response by down-regulating interferon-stimulated genes (ISGs) expression: acts by binding to FOXO3 mRNAs. 1. Shi, H. et al. (2017) Cell research 27, 315-328. PMID: 28106072 2. Li, A. et al. (2017) Cell research 27, 444-447. PMID: 28106076 3. Yang, Y. et al. (2017) Cell research 27, 626-641. PMID: 28281539 4. Zaccara, S. et al. (2020) Cell 181, 1582-1595.e18. PMID: 32492408 5. Dominissini, D. et al. (2012) Nature 485, 201-6. PMID: 22575960 6. Wang, X. et al. (2014) Nature 505, 117-20. PMID: 24284625 7. Ries, RJ. et al. (2019) Nature 571, 424-428. PMID: 31292544
Note	For research use only.

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SDS-PAGE for Recombinant Human YTHDF3 Protein, N-His

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Datasheet

Human YTHDF3 Recombinant Protein (N-His).pdf

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Human YTHDF3 Recombinant Protein (N-His) (HA146012)

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