Human ZNF410 Recombinant Protein (N-His) (HP086012)

Name: Human ZNF410 Recombinant Protein (N-His)
Brand: abinScience
SKU: HP086012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HP086012
Description	Recombinant Human ZNF410 Protein, N-His (HP086012) expressed in E. coli, spanning Arg76-Ser191. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q86VK4
Protein length	Arg76-Ser191
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	14.83 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	ZNF410, APA1, Another partner for ARF 1, Zinc finger protein 410, Zinc finger protein APA-1
Background	Zinc finger protein 410 (ZNF410) is a ~52 kDa protein. Transcription factor that binds to the sequence motif 5'-CATCCCATAATA-3', and is specifically required to silence expression of fetal hemoglobin in adult erythroid cells. Prevents expression of fetal hemoglobin genes HBG1 and HBG2 through CHD4: acts as a direct transcriptional activator of CHD4, a central component of the NuRD complex that represses transcription of fetal hemoglobin genes HBG1 and HBG2 in erythroid cells. May also activate transcription of matrix-remodeling genes such as MMP1 during fibroblast senescence. May activate transcription of the gap junction gene GJC1, perhaps in response to increasing glucose. However, recent studies suggest that ZNF410 is dedicated to regulate expression of a single gene: CHD4. 1. Lan, X. et al. (2021) Molecular cell 81, 239-254.e8. PMID: 33301730 2. Vinjamur, DS. et al. (2021) Nature genetics 53, 719-728. PMID: 33859416 3. Benanti, JA. et al. (2002) Molecular and cellular biology 22, 7385-97. PMID: 12370286 4. Chen, Y. et al. (2018) Journal of cellular physiology 234, 606-618. PMID: 30078215
Note	For research use only.

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SDS-PAGE for Recombinant Human ZNF410 Protein, N-His

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Datasheet

Human ZNF410 Recombinant Protein (N-His).pdf

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Human ZNF410 Recombinant Protein (N-His) (HP086012)

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