Mouse CCN3 Recombinant Protein (N-His-SUMO) (MW350012)

Name: Mouse CCN3 Recombinant Protein (N-His-SUMO)
Brand: abinScience
SKU: MW350012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	MW350012
Description	Recombinant Mouse CCN3 Protein, N-His-SUMO (MW350012) expressed in E. coli, spanning Ser50-Arg137. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q64299
Protein length	Ser50-Arg137
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Mus musculus (Mouse)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	22.00 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	NovH, Cellular communication network factor 3, CCN family member 3, Nephroblastoma-overexpressed gene protein homolog, Protein NOV homolog, Nov, Ccn3
Background	CCN family member 3 (CCN3) is a ~38 kDa protein. Immediate-early protein playing a role in various cellular processes including proliferation, adhesion, migration, differentiation and survival. Acts by binding to integrins or membrane receptors such as NOTCH1. Essential regulator of hematopoietic stem and progenitor cell function. Inhibits myogenic differentiation through the activation of Notch-signaling pathway. Inhibits vascular smooth muscle cells proliferation by increasing expression of cell-cycle regulators such as CDKN2B or CDKN1A independently of TGFB1 signaling. 1. Matsushita, Y. et al. (2013) The Journal of biological chemistry 288, 19973-85. PMID: 23653360 2. Paradis, R. et al. (2013) PloS one 8, e64957. PMID: 23705021 3. Liu, J. et al. (2014) PloS one 9, e94912. PMID: 24722330 4. Babey, ME. et al. (2024) Nature 632, 357-365. PMID: 38987585
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Mouse CCN3 Protein, N-His-SUMO

References

MW350012-SDS-PAGE-1.jpg

Datasheet

Mouse CCN3 Recombinant Protein (N-His-SUMO).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Mouse CCN3 Recombinant Protein (N-His-SUMO) (MW350012)

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