Mouse Semaphorin 4D/CD100 Recombinant Protein (C-His) (MV563011)

Name: Mouse Semaphorin 4D/CD100 Recombinant Protein (C-His)
Brand: abinScience
SKU: MV563011
Price: 298 USD
Availability: InStock

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Overview

Catalog No.	MV563011
Description	Recombinant Mouse CD100/SEMA4D Protein, C-His (MV563011) expressed in Mammalian Cells, spanning Met1-Arg733. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●Mammalian Expression — Proper folding and native glycosylation. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	Mammalian cells
Accession	O09126
Protein length	Met1-Arg733
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Mus musculus (Mouse)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Predicted molecular weight	82.21 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Semaphorin-4D, M-Sema G, Semaphorin-C-like 2, Semaphorin-J, Sema J, CD100, Sema4d, Semacl2, Semaj
Background	Semaphorin-4D is a ~95 kDa protein. Cell surface receptor for PLXNB1 and PLXNB2 that plays an important role in cell-cell signaling. Regulates GABAergic synapse development. Promotes the development of inhibitory synapses in a PLXNB1-dependent manner. Modulates the complexity and arborization of developing neurites in hippocampal neurons by activating PLXNB1 and interaction with PLXNB1 mediates activation of RHOA. Promotes the migration of cerebellar granule cells. 1. Kuzirian, MS. et al. (2013) The Journal of neuroscience : the official journal of the Society for Neuroscience 33, 8961-73. PMID: 23699507 2. McDermott, JE. et al. (2018) Molecular and cellular neurosciences 92, 50-66. PMID: 29981480 3. Deng, S. et al. (2007) The Journal of neuroscience : the official journal of the Society for Neuroscience 27, 6333-47. PMID: 17554007
Note	For research use only

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SDS-PAGE

SDS-PAGE for Recombinant Mouse CD100/SEMA4D Protein, C-His

References

Datasheet

Mouse Semaphorin 4D/CD100 Recombinant Protein (C-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

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Working Solution

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Mouse Semaphorin 4D/CD100 Recombinant Protein (C-His) (MV563011)

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