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Mouse CD150/SLAMF1 Recombinant Protein (C-His) (MC275011)

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Overview
Catalog No.MC275011
Description
Recombinant Mouse CD150/SLAMF1 Protein, C-His (MC275011) expressed in Mammalian Cells, spanning Thr25-Pro242. Purity: >90% by SDS-PAGE.
Highlights
  • His-Tagged — N-terminal 6×His tag for IMAC purification.
  • Mammalian Expression — Proper folding and native glycosylation.
  • High Purity — >90% purity verified by SDS-PAGE.
Expression systemMammalian cells
AccessionQ9QUM4
Protein lengthThr25-Pro242
ApplicationsELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
SpeciesMus musculus (Mouse)
Nature Recombinant
Endotoxin level Please contact with the lab for this information.
Purity >90% as determined by SDS-PAGE.
Predicted molecular weight 27.09 kDa
Form Lyophilized
Storage buffer Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate NamesCD150, CDw150, IPO-3, SLAM, SLAM family member 1, SLAMF1, Signaling lymphocytic activation molecule
Background

Signaling lymphocytic activation molecule is a ~38 kDa protein. Self-ligand receptor of the signaling lymphocytic activation molecule (SLAM) family. SLAM receptors triggered by homo- or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are involved in the regulation and interconnection of both innate and adaptive immune response. Activities are controlled by presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2. SLAMF1-induced signal-transduction events in T-lymphocytes are different from those in B-cells. Two modes of SLAMF1 signaling seem to exist: one depending on SH2D1A (and perhaps SH2D1B) and another in which protein-tyrosine phosphatase 2C (PTPN11)-dependent signal transduction operates.

1. Howie, D. et al. (2002) Blood 100, 2899-907. PMID: 12351401
2. Aversa, G. et al. (1997) Journal of immunology (Baltimore, Md. : 1950) 158, 4036-44. PMID: 9126961
3. Latour, S. et al. (2001) Nature immunology 2, 681-90. PMID: 11477403
4. Cannons, JL. et al. (2004) Immunity 21, 693-706. PMID: 15539155
5. Chen, R. et al. (2006) Molecular and cellular biology 26, 5559-68. PMID: 16847311
6. Wang, N. et al. (2004) The Journal of experimental medicine 199, 1255-64. PMID: 15123745
7. Yusuf, I. et al. (2010) Journal of immunology (Baltimore, Md. : 1950) 185, 190-202. PMID: 20525889
8. Wang, N. et al. (2006) American journal of respiratory cell and molecular biology 35, 206-10. PMID: 16528012
9. Griewank, K. et al. (2007) Immunity 27, 751-62. PMID: 18031695
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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