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Mouse CD300A Recombinant Protein (C-Fc) (MV400021)

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Overview
Catalog No.MV400021
Description
Recombinant Mouse CD300A Protein, C-Fc (MV400021) expressed in Mammalian Cells, spanning Leu28-Pro185. Purity: >90% by SDS-PAGE.
Highlights
  • Fc Fusion — Enhanced stability with Protein A/G purification compatibility.
  • Mammalian Expression — Proper folding and native glycosylation.
  • High Purity — >90% purity verified by SDS-PAGE.
Expression systemMammalian cells
AccessionQ6SJQ0
Protein lengthLeu28-Pro185
ApplicationsELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
SpeciesMus musculus (Mouse)
Nature Recombinant
Endotoxin level Please contact with the lab for this information.
Purity >90% as determined by SDS-PAGE.
Predicted molecular weight 45.69 kDa
Form Lyophilized
Storage buffer Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate NamesCD300 antigen-like family member A, CD300A, CD300a, CLM-8, CMRF-35-H9, CMRF35-H, CMRF35-H9, CMRF35-like molecule 8, CMRF35H, IGSF12, IRC1/IRC2, IRp60, IgSF12, Immunoglobulin superfamily member 12, Inhibitory receptor protein 60, NK inhibitory receptor
Background

CMRF35-like molecule 8 is a ~35 kDa protein. Inhibitory receptor which may contribute to the down-regulation of cytolytic activity in natural killer (NK) cells, and to the down-regulation of mast cell degranulation. Negatively regulates the Toll-like receptor (TLR) signaling mediated by MYD88 but not TRIF through activation of PTPN6.

1. Yotsumoto, K. et al. (2003) The Journal of experimental medicine 198, 223-33. PMID: 12874256
2. Kumagai, H. et al. (2003) Biochemical and biophysical research communications 307, 719-29. PMID: 12893283
3. Bachelet, I. et al. (2005) Journal of immunology (Baltimore, Md. : 1950) 175, 7989-95. PMID: 16339535
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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Volume
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Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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