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Recombinant Mouse DKK1 Protein, N-His (MF914012)

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Recombinant Mouse DKK1 Protein, N-His

Recombinant Mouse DKK1 Protein, N-His

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Datasheet

Overview

Catalog No.	MF914012
Description	Recombinant Mouse DKK1 Protein, N-His (MF914012) expressed in E. coli, spanning Ile184-His272. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	O54908
Protein length	Ile184-His272
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Mus musculus (Mouse)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	12.33 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Dickkopf-1, SK, hDkk-1, Dkk-1, Dickkopf-related protein 1, DKK1
Background	Dickkopf-related protein 1 (DKK1) is a ~29 kDa protein. Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. Inhibits the pro-apoptotic function of KREMEN1 in a Wnt-independent manner, and has anti-apoptotic activity. Plays a role in limb development; attenuates Wnt signaling in the developing limb to allow normal limb patterning. 1. Chen, L. et al. (2008) The Journal of biological chemistry 283, 23364-70. PMID: 18524778 2. Causeret, F. et al. (2016) Cell death and differentiation 23, 323-32. PMID: 26206087 3. Ellwanger, K. et al. (2008) Molecular and cellular biology 28, 4875-82. PMID: 18505822
Note	For research use only.

Images

SDS-PAGE

SDS-PAGE for Recombinant Mouse DKK1 Protein, N-His

References

MF914012-SDS-PAGE-1.jpg

Datasheet

Recombinant Mouse DKK1 Protein, N-His.pdf

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Recombinant Mouse DKK1 Protein, N-His