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Mouse FGA Recombinant Protein (N-His) (MF809012)

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Overview
Catalog No.MF809012
Description
Recombinant Mouse FGA Protein, N-His (MF809012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE..
Highlights
  • E. coli Expression — High-yield, cost-effective production.
  • High Purity — >90% as determined by SDS-PAGE.
Expression systemE. coli
AccessionE9PV24
Protein lengthThr552-Gln789
ApplicationsELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
SpeciesMus musculus (Mouse)
Nature Recombinant
Endotoxin level Please contact with the lab for this information.
Purity >90% as determined by SDS-PAGE.
Form Lyophilized
Storage buffer Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate NamesFga, Fibrinogen alpha chain, Fibrinopeptide A
Background

Fibrinogen alpha chain is a ~87 kDa protein. Cleaved by the protease thrombin to yield monomers which, together with fibrinogen beta (FGB) and fibrinogen gamma (FGG), polymerize to form an insoluble fibrin matrix. Fibrin has a major function in hemostasis as one of the primary components of blood clots. In addition, functions during the early stages of wound repair to stabilize the lesion and guide cell migration during re-epithelialization. Was originally thought to be essential for platelet aggregation, based on in vitro studies using anticoagulated blood. However, subsequent studies have shown that it is not absolutely required for thrombus formation in vivo.

1. Suh, TT. et al. (1995) Genes & development 9, 2020-33. PMID: 7649481
2. Drew, AF. et al. (2001) Blood 97, 3691-8. PMID: 11389004
3. Ni, H. et al. (2000) The Journal of clinical investigation 106, 385-92. PMID: 10930441
4. Yang, H. et al. (2009) Blood 114, 425-36. PMID: 19332769
5. Johnson, LL. et al. (2003) The Journal of experimental medicine 197, 801-6. PMID: 12629066
6. Luo, D. et al. (2013) Journal of immunology (Baltimore, Md. : 1950) 190, 4149-61. PMID: 23487423
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
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C₁ (Stock Conc.)
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