Mouse IL22 Recombinant Protein (N-His) (MV999012)

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Catalog No.	MV999012
Description	Recombinant Mouse IL22 Protein, N-His (MV999012) expressed in E. coli, spanning Leu34-Val179. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q9JJY9
Protein length	Leu34-Val179
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Mus musculus (Mouse)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Interleukin-22, IL-22, IL-10-related T-cell-derived-inducible factor, IL-TIF, IL-TIF alpha, Interleukin-22a, IL-22a, Il22, Il22a, Iltif, Iltifa
Background	Interleukin-22 is a ~20 kDa protein. Cytokine that plays a critical role in modulating tissue responses during inflammation. Plays an essential role in the regeneration of epithelial cells to maintain barrier function after injury and for the prevention of further tissue damage. Unlike most of the cytokines, has no effect on immune cells. Signals through a heterodimeric receptor composed of two subunits, the specific receptor IL22RA1 which is present on non-immune cells in many organs and the shared subunit IL10RB. Ligation of IL22RA1 with IL22 induces activation of the tyrosine kinases JAK1 and TYK2, which in turn activates STAT3. 1. Saxton, RA. et al. (2021) Immunity 54, 660-672.e9. PMID: 33852830 2. Ulu, A. et al. (2022) Toxicology and applied pharmacology 446, 116044. PMID: 35525330 3. Delbue, D. et al. (2021) Frontiers in medicine 8, 656047. PMID: 33912578 4. Weathington, NM. et al. (2014) The Journal of biological chemistry 289, 17610-9. PMID: 24742671
Note	For research use only

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SDS-PAGE for Recombinant Mouse IL22 Protein, N-His

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Datasheet

Mouse IL22 Recombinant Protein (N-His).pdf

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Mouse IL22 Recombinant Protein (N-His) (MV999012)

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