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Mouse SORD Recombinant Protein (N-His) (MX841012)

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Overview
Catalog No.MX841012
Description
Recombinant Mouse SORD Protein, N-His (MX841012) expressed in E. coli, spanning Met1-Pro357. Purity: >90% by SDS-PAGE.
Highlights
  • His-Tagged — N-terminal 6×His tag for IMAC purification.
  • E. coli Expression — High-yield, cost-effective production.
  • High Purity — >90% purity verified by SDS-PAGE.
Expression systemE. coli
AccessionQ64442
Protein lengthMet1-Pro357
ApplicationsELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
SpeciesMus musculus (Mouse)
Nature Recombinant
Endotoxin level Please contact with the lab for this information.
Purity >90% as determined by SDS-PAGE.
Predicted molecular weight 40.41 kDa
Form Lyophilized
Storage buffer Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names(R,R)-butanediol dehydrogenase, EC:1.1.1.-, EC:1.1.1.14, EC:1.1.1.4, EC:1.1.1.56, EC:1.1.1.9, L-iditol 2-dehydrogenase, Polyol dehydrogenase, RDH, Ribitol dehydrogenase, SDH, SORD, Sorbitol dehydrogenase, XDH, Xylitol dehydrogenase
Background

Sorbitol dehydrogenase is a ~38 kDa protein. Polyol dehydrogenase that catalyzes the reversible NAD(+)-dependent oxidation of various sugar alcohols. Is active with D-sorbitol (D-glucitol) leading to the C2-oxidized product D-fructose. Is a key enzyme in the polyol pathway that interconverts glucose and fructose via sorbitol, which constitutes an important alternate route for glucose metabolism. May play a role in sperm motility by using sorbitol as an alternative energy source for sperm motility and protein tyrosine phosphorylation. Has no activity on ethanol.

1. Burnell, JN. et al. (1983) The International journal of biochemistry 15, 507-11. PMID: 6852349
2. Cao, W. et al. (2009) Biology of reproduction 80, 124-33. PMID: 18799757
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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