ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Aedes aegypti (Yellowfever mosquito)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Predicted molecular weight
36.19 kDa
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names
Long form salivary protein D7L1, AeD7, AeD7L1, 37 kDa salivary gland allergen Aed a 2, Protein D7, Aed a 2, D7, D7L1, AAEL006424, Aed a 2
Background
Long form salivary protein D7L1 (Aed a 2/AeD7L1) is a ~36 kDa protein. Modulates blood feeding of female mosquitoes on vertebrate species by binding and sequestering different mediators involved in the host response, such as biogenic amines and eicosanoids. Binds serotonin, histamine, leukotriene B4, leukotriene C4, leukotriene D4, leukotriene E4, adrenaline and noradrenaline. Does not bind tryptamine and U-46619, a stable analog of thromboxane A2. Exhibits vasodilating activity. Inhibits agonist-induced platelet aggregation but not blood clotting.
1. Martin-Martin, I. et al. (2021) The FEBS journal 288, 2014-2029. PMID: 32799410
2. Martin-Martin, I. et al. (2023) mBio 14, e0228923. PMID: 37909749
3. Calvo, E. et al. (2006) The Journal of biological chemistry 281, 1935-42. PMID: 16301315
4. Calvo, E. et al. (2009) Proceedings of the National Academy of Sciences of the United States of America 106, 3728-33. PMID: 19234127
Note
For research use only.
Images
SDS-PAGE
SDS-PAGE for Recombinant Yellowfever mosquito Aed a 2/AeD7L1 Protein, C-His
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