Anti-Human TRAIL R2/TNFRSF10B Reference Antibody (TAS266, RUO) (HT348076)

Name: Anti-Human TRAIL R2/TNFRSF10B Reference Antibody (TAS266, RUO)
Brand: abinScience
SKU: HT348076
Price: 176 USD
Availability: InStock

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Overview

Catalog No.	HT348076
Species reactivity	Human
Applications	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Host species	Human
Isotype	(VHH-VHH-hFc)-dimer
Clone ID	TAS266
Biological activity	TAS266 is up to 1000-fold more potent than cross-linked DR5 antibody or TRAIL at inducing cell death in cancer cell lines via DR5 activation, with faster downstream caspase activation kinetics [1]. TAS266 potently induces death in human tumor cell lines, with potency ≥1000-fold greater than cross-linked DR5 antibody LBY135 or TRAIL [2]. TAS266 (200 mg/kg; twice weekly) shows no target organ toxicity including hepatotoxicity in cynomolgus monkeys after administration, with its biological activity remaining intact [2]. TAS266 (single dose; weekly) induces tumor regression in a variety of tumor xenograft models, and its antitumor activity with weekly administration is superior to that of DR5 agonist antibodies in pancreatic cancer and colon cancer xenograft models [2]. . Maintained bioactivity despite presence of preexisting or treatment-emergent TAS266-binding IgG antibodies in some animals. . Exhibited superior anti-tumor activity compared with a DR5 agonist antibody in patient-derived pancreatic and colon xenograft models.
Expression system	Mammalian cells
Clonality	Monoclonal
Target	TRAILR2, TRAIL-R2, Death receptor 5, CD262, DR5, Tumor necrosis factor receptor superfamily member 10B, ZTNFR9, TRAIL receptor 2, TRICK2, TNFRSF10B, KILLER, TNF-related apoptosis-inducing ligand receptor 2
Endotoxin level	Please contact the lab for this information.
Purity	>95% purity as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	O14763
Form	Liquid
Storage buffer	0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	TAS266
Background	Tumor necrosis factor receptor superfamily member 10B (CD262/TRAIL-R2) is a ~47 kDa protein. Receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF-kappa-B. Essential for ER stress-induced apoptosis. 1. Hymowitz, SG. et al. (1999) Molecular cell 4, 563-71. PMID: 10549288
References	1. Huet H, et al. TAS266, a novel tetrameric nanobody agonist targeting death receptor 5 (DR5), elicits superior antitumor efficacy than conventional DR5-targeted approaches[J]. Cancer Research, 2012, 72(8_Supplement): 3853-3853. [HT348076] 2. Papadopoulos KP, et al. Unexpected hepatotoxicity in a phase I study of TAS266, a novel tetravalent agonistic Nanobody® targeting the DR5 receptor. Cancer Chemother Pharmacol. 2015;75(5):887-895. [HT348076]
Note	For research use only. Not suitable for clinical or therapeutic use.

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Bioactivity

Detects Human CD262/TNFRSF10B/TRAIL-R2/DR5 in indirect ELISAs.
SDS-PAGE

SDS-PAGE for Research Grade Anti-Human CD262/TNFRSF10B/TRAIL-R2/DR5 (TAS266)
Western blot

Western blot analysis was performed using anti-CD262/TNFRSF10B/DR5 monoclonal antibody at 1μg/ml on various samples.
Lane 1: recombinant human CD262/TNFRSF10B/DR5 (Catalog No: HT348021), 50ng
Lane 2: recombinant human CD262/TNFRSF10B/DR5 (Catalog No: HT348021), 30ng

References

Datasheet

Anti-Human TRAIL R2/TNFRSF10B Reference Antibody (TAS266, RUO).pdf

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Anti-Human TRAIL R2/TNFRSF10B Reference Antibody (TAS266, RUO) (HT348076)

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