Anti-Human IL1RAP Reference Antibody (CAN10, RUO) (HV556026)

Name: Anti-Human IL1RAP Reference Antibody (CAN10, RUO)
Brand: abinScience
SKU: HV556026
Price: 176 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.	HV556026
Species reactivity	Human
Applications	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Host species	Human
Isotype	IgG1-kappa
Clone ID	CAN10
Biological activity	CAN10 effectively impairs the proinflammatory and profibrotic effects of IL1RAP-related cytokines (IL-1, IL-33, and IL-36) in human umbilical cord endothelial cells and human dermal fibroblasts, which are critical to systemic sclerosis pathophysiology [1]. CAN10 (mCAN10, mouse surrogate of CAN10) (10-20 mg/kg; i.p.; twice weekly; 7 weeks) reduces cutaneous and pulmonary fibrosis in a murine cGvHD model of systemic sclerosis, with significant improvements in dermal thickness, hydroxyproline content, myofibroblast counts, and lung Ashcroft scores [1].
Expression system	Mammalian cells
Clonality	Monoclonal
Target	IL-1R3, C3orf13, Interleukin-1 receptor 3, Interleukin-1 receptor accessory protein, IL1RAP, IL-1R-3, IL-1 receptor accessory protein, IL1R3, IL-1RAcP
Endotoxin level	Please contact the lab for this information.
Purity	>95% purity as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	Q9NPH3
Form	Liquid
Storage buffer	0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	CAN10
Background	Interleukin-1 receptor accessory protein (IL1RAP) is a ~65 kDa protein. Coreceptor for IL1RL2 in the IL-36 signaling system. Coreceptor with IL1R1 in the IL-1 signaling system. Associates with IL1R1 bound to IL1B to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B and other pathways. Signaling involves the recruitment of adapter molecules such as TOLLIP, MYD88, and IRAK1 or IRAK2 via the respective TIR domains of the receptor/coreceptor subunits. Recruits TOLLIP to the signaling complex.
References	1. Grönberg C, et al. Combined inhibition of IL-1, IL-33 and IL-36 signalling by targeting IL1RAP ameliorates skin and lung fibrosis in preclinical models of systemic sclerosis. Ann Rheum Dis. 2024;83(9):1156-1168. Published 2024 Aug 27. [HV556026]
Note	For research use only. Not suitable for clinical or therapeutic use.

Images

SDS-PAGE

SDS-PAGE for Research Grade Anti-Human IL1RAP (CAN10)
Bioactivity

Detects Recombinant Human IL1RAP Protein, N-His (Catalog No: HV556011) in indirect ELISAs.
Western blot

Western blot analysis was performed using anti-IL1RAP monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human IL1RAP (Catalog No: HV556011)
Lane 2: negative control
Lane 3: negative control