Anti-Human TNFSF15/TL1A & TNFa/TNF-alpha Reference Antibody (AMG 966, RUO) (HF526066)

Name: Anti-Human TNFSF15/TL1A & TNFa/TNF-alpha Reference Antibody (AMG 966, RUO)
Brand: abinScience
SKU: HF526066
Price: 176 USD
Availability: InStock

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Overview

Catalog No.	HF526066
Species reactivity	Human
Applications	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Host species	Human
Isotype	IgG1-kappa
Clone ID	AMG 966
Biological activity	AMG-966 (300 nM; 6 days) elicits minimal CD4+ T cell proliferation in a human DC:T cell assay from 30 global HLA-representative donors, with no impact from its engineered charge pair mutations [1]. AMG-966 (1:1, 1:1:1, 9:1:1, 200:1:1, 600:1:1 molar ratios; approximately 16 hours) forms intermediate-sized immune complexes with recombinant human TNF at a 1:1 ratio and larger complexes when combined with both TNF and TL1A at lower drug-to-target ratios, as measured by SEC-MALS after 16-hour incubation at 4°C [1]. AMG-966 (1:1:1, 4:1:1, 9:1:1, 100:1:1 molar ratios; overnight) forms immune complexes with recombinant human TNF and TL1A that exhibit enhanced binding to FcγRIa and FcγRIIa at low 1:1:1 drug-to-target ratios (after overnight incubation at 4°C), with binding decreasing as the drug-to-target ratio increases, while FcγRIIIA binding is minimally affected [1].
Expression system	Mammalian cells
Clonality	Monoclonal
Target	TNFSF15, Vascular endothelial cell growth inhibitor, VEGI, TL1, TNF ligand-related molecule 1, Tumor necrosis factor ligand superfamily member 15, TNF, Tumor necrosis factor ligand superfamily member 2, N-terminal fragment, ICD2, NTF, TNF-a, TNF-alpha, Tumor necrosis factor, TNFSF2, TNFA, Cachectin, ICD1
Endotoxin level	Please contact the lab for this information.
Purity	>95% purity as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	O95150 & P01375
Form	Liquid
Storage buffer	0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Bispecific, AMG966, AMG-966
References	1. Kroenke MA, et al. Immune Complex Formation Is Associated With Loss of Tolerance and an Antibody Response to Both Drug and Target. Front Immunol. 2021;12:782788. Published 2021 Dec 14. [HF526066]
Note	For research use only. Not suitable for clinical or therapeutic use.

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SDS-PAGE

SDS-PAGE for Anti-Human TNFSF15/TL1A & TNFa/TNF-alpha Reference Antibody (AMG 966, RUO)
Bioactivity

Detects recombinant human TNFa (Cat No.: HF879011) in indirect ELISA.
Bioactivity

Detects recombinant human TNFa (Cat No.: HF526011) in indirect ELISA.
Bioactivity

Detects Recombinant Human TNFSF15/TL1A Protein, C-His (Cat No.: HF526011) in indirect ELISA.
Flow CytoMetry

Flow-cytometry APC using anti-human TNFa antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an APC anti-human TNFa monoclonal antibody (Catalog: HF526066) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry FITC using anti-human TNFa antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an FITC anti-human TNFa monoclonal antibody (Catalog: HF526066) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using anti-human TNFa antibody. 293T Transtected cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human TNFa monoclonal antibody (Catalog: HF526066, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Western blot

Western blot analysis was performed using anti-TNFSF15/TL1A monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human TNFSF15/TL1A (Catalog No: HF526011)
Lane 2: negative control

References

Datasheet

Anti-Human TNFSF15/TL1A & TNFa/TNF-alpha Reference Antibody (AMG 966, RUO).pdf

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Anti-Human TNFSF15/TL1A & TNFa/TNF-alpha Reference Antibody (AMG 966, RUO) (HF526066)

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Datasheet

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