Anti-Human EphA3 Reference Antibody (Ifabotuzumab, RUO) (HB681016)

Name: Anti-Human EphA3 Reference Antibody (Ifabotuzumab, RUO)
Brand: abinScience
SKU: HB681016
Price: 176 USD
Availability: InStock

Datasheet Share product Copy info

Price(USD):

Spec:

100ug
1mg
5mg
10mg
25mg
50mg
100mg

Number:

Get quote

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.	HB681016
Description	Ifabotuzumab (HB681016) is a research-grade recombinant antibody targeting HEK. Produced in mammalian cells with native-like glycosylation. Highlights ●Research Grade — For PK/PD studies, assay development, and ADA research. ●Native Glycosylation — Mammalian expression ensures native-like patterns.
Species reactivity	Human
Applications	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Host species	Human
Isotype	IgG1-kappa
Expression system	Mammalian cells
Clonality	Monoclonal
Target	HEK, Eph-like tyrosine kinase 1, EK4, EPHA3, Tyrosine-protein kinase receptor ETK1, Tyrosine-protein kinase TYRO4, Ephrin type-A receptor 3, hEK4, ETK, TYRO4, ETK1, Human embryo kinase, EPH-like kinase 4
Endotoxin level	Please contact the lab for this information.
Purity	>95% purity as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P29320
Form	Liquid
Storage buffer	0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Fibatuzumab, KB-004, 1234137-51-9
Background	Ephrin type-A receptor 3 (EPHA3) is a ~110 kDa protein. Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Highly promiscuous for ephrin-A ligands it binds preferentially EFNA5. Upon activation by EFNA5 regulates cell-cell adhesion, cytoskeletal organization and cell migration. Also activated by EFNA1, inhibiting epithelial-to-mesenchymal transition of cardiac cells and playing a role in heart development. 1. Lawrenson, ID. et al. (2002) Journal of cell science 115, 1059-72. PMID: 11870224 2. Sharfe, N. et al. (2003) Journal of immunology (Baltimore, Md. : 1950) 170, 6024-32. PMID: 12794130
Note	For research use only. Not suitable for clinical or therapeutic use.

Images

SEC-HPLC

SEC-HPLC detection for Research Grade Ifabotuzumab.
Bioactivity

Detects EPHA3 in indirect ELISAs.
SDS-PAGE

SDS-PAGE for Research Grade Ifabotuzumab.
Flow Cytometry

Flow-cytometry using anti-human EPHA3 antibody. Jurkat cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human EPHA3 monoclonal antibody (Catalog HB681016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet

Anti-Human EphA3 Reference Antibody (Ifabotuzumab, RUO).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human EphA3 Reference Antibody (Ifabotuzumab, RUO) (HB681016)

Overview

Images

References

Datasheet

SDS

Quick access

Contact Us