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Research Grade Insulin Aspart (HF990026)

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Overview
Catalog No.HF990026
Species reactivityHuman
ApplicationsELISA, Functional assay, Research in vivo
IsotypeRecombinant human Insulin analogue
Biological activity Insulin cattle is the two-chain polypeptide hormone produced by the β-cells of pancreatic islets. The α and β chains are joined by two interchain disulfide bonds. The α chain contains an intrachain disulfide bond. Insulin regulates glucose uptake into muscle and fat cells by recruiting membrane glucose transporter Glut-4 to cell surface. Insulin cattle has often been used as growth supplement in culturing cells at the concentration ranging from 1 to 10 ug/mL of medium [1]. in the pancreatic β cells. Insulin cattle has often been used as growth supplement in culturing cells.
Expression system Mammalian cells
Target INS, Insulin
Endotoxin level Please contact the lab for this information.
Purity >95% purity as determined by SDS-PAGE.
Purification Purified by Nickel column.
Accession P01308
Form Liquid
Storage buffer 0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Insulin is a ~11 kDa protein. Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.

References 1. Yousefi R, et al. Aspirin-mediated acetylation induces structural alteration and aggregation of bovine pancreaticinsulin. J Biomol Struct Dyn. 2016;34(2):362-75. [HF990026]
Note For research use only. Not suitable for clinical or therapeutic use.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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